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For review, the images you upload will automatically be converted to PDF and HTML for reviewers to download. Please label and number your figures and figure legends clearly. Each figure should be given a short heading, which will be published in bold font.
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Reducing or increasing contrast to hide data is forbidden. Processing to emphasize a region in an image to the detriment of other regions is inappropriate, particularly where an attempt is made to reinforce significance of experimental data relative to the control. All images must accurately represent the original data in line with the latest standards now expected by the research community. Please list in the ‘Materials and methods’ section all tools and software used to acquire images, including image gathering settings and any required processing manipulations. Avoid using different shades of grey or colours that are close in hue to identify different symbols or columns in a bar chart.
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Also ensure that the presentation will work on any internet browser. Brain Communicationsallows cropped gels and blots in the main paper only if they improve understanding of the data reported. Cropping must be indicated in the image and mentioned in the figure legend. Full-length gels and/or blots are to be made available as Supplementary material, which will be published online if the manuscript is accepted.
Symbols and lines should be distinguishable when the figure is reduced, and no smaller than 5 and 0.5 pt, respectively at the final size. For useful information on preparing your figures for publication, clickhere.
Use of cloning and healing tools, such as those available in Photoshop, or any feature that deliberately obscures manipulations, is considered inappropriate. Where such tools are used by necessity, for example to remove identifying data about patients from an image, then this should be explicitly mentioned in the figure legend. Changing contrast or brightness may be acceptable when it is applied evenly over the whole image including over the controls.
Colour should be used sparingly to identify different categories of data, and red and green should not be used together in graphs. Continuously distributed data should be displayed either by showing all data points or by using box-and-whisker plots, with all elements (e.g. median, interquartile interval, minimum, maximum) defined in the legend. Solid symbols are preferable to open symbols except to indicate data overlap. Circles, squares, diamonds and triangles are preferable to crosses.
We recommend consolidating multiple supplementary elements into a single file where this would make the reader/reviewer experience simpler. If possible, please combine all supplementary data files in a single PDF file. Please note that Supplementary material download free software will not be edited, so ensure that it is clearly and succinctly presented, that the style of terms conforms with the rest of the paper, and that there are no tracked changes.